Stability Indicating Assay Method by UV Spectrophotometryfor Estimation of Ofloxacin and Flavoxate Hydrochloride inTablet Dosage Form

Abstract – The present research work discusses
the development of a stability-indicating UV
spectrophotometric method for the estimation
of Ofloxacin and Flavoxate hydrochloride in
tablet dosage form. The optimum conditions
for the analysis of the drug were established.
The maximum wavelength (λmax ) was found
to be 266 nm for ofloxacin and 272 nm for
flavoxate. Degradation studies of ofloxacin and
flavoxate showed prominent degradation in acid
hydrolysis.
Key words – Stability Indicating, Ofloxacin,
Flavoxate, UV spectrophotometry.
I. INTRODUCTION
Ofloxacin (OFL) is 9-fluro-2,3-dihydro-3-
methyl-10-(4-methyl-1-piperazin-yl)-7-oxo-
7H-pyridol[1,2,3-de]-1,4,-benzoxazine-6-
carboxylic acid. It is a member of quinolone
class of antibacterial drugs wherein the 1 and 8
positions are joined in the form of 1,4-oxazine
ring. This quinolone is also widely distributed
into most body fluids and tissues. Higher
concentration of ofloxacin is achieved in CSF
than can be obtained with ciprofloxacin. The
oral bioavailability of ofloxacin is 95% to 100%.
The amount of an administered dose of ofloxacin
excreted in the urine in a 24 – 48 hour period
range from 70% to 90%. There relatively little
biliary excretion of this drug. The elimination
half life of ofloxacin ranges from 4.5 to 7 hours.
[1] Flavoxate is indicated in urinary frequency,
urgency and dysuria associated with lower
urinary tract infection. It is an anti-muscarinic,
has high affinity for receptors in urinary bladder
and salivary glands with additional smooth
muscle relaxant and local anaesthetic properties.
It is relatively selective for M1/M3 subtypes than
for M2. Because of vasicoselective action it is used
for instability resulting in urinary frequency and
urge incontinence.